Standardize the Measurement.
Scale the Science.

Cytely turns biological data into standardized, reproducible measurement. Go from sample to auditable results faster, with higher quality and unparalleled ease.

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Ready-to-go assays
Reproducible results
Cytely interface dashboard showing data-driven microscopy

How many of my cells have taken up the prey?

Are different cell phenotypes driving distinct uptake patterns?

How much of the prey is associated with cells and how much is outside?

Are the cells that have phagocytosed the prey evenly spread out?

Are the cells that have taken up the most prey morphologically different from other cells?

How evenly is the prey phagocytosed?

How can I image rare events without missing them?

How do I ensure my analysis is unbiased and reproducible?

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Analysis in Minutes, Not Weeks

Analysis in Minutes, Not Weeks

Forget complex parameter tuning and building analysis pipelines. Cytely automatically identifies the cells and objects in your sample with minimal configuration. Simply drag-and-drop your images and define your high-level goals; Cytely handles the complex setup. This contrasts sharply with tools requiring extensive user expertise. Cytely's approach significantly simplifies advanced microscopy, making it accessible to more researchers by removing the need for deep knowledge of both microscope hardware and complex analysis software like ImageJ or CellProfiler.

Explore Instantly & Interactively

Explore Instantly & Interactively

Cytely goes beyond data collection: it turns complex image data into immediate insight. Once analysis is complete, you're presented with a fully interactive interface: scatter plots of extracted features, a panel of cropped cell images, and a spatial map showing each object's position within the sample. Define populations by drawing gates directly on the scatter plots. As you do, both the image gallery and spatial map update in real time to reflect your selection. Every data point is a cell you can see, not just a number. All extracted features, including intensity, size, and shape, are instantly available for plotting. Gates can be added, renamed, and reused without reprocessing. No scripts. No pipelines. Just visual, intuitive, exploratory analysis.

Works With Your Existing Equipment

Works With Your Existing Equipment

Continue using your existing microscope setup. Cytely integrates seamlessly into your imaging workflow: scan broadly, upload your data, and let Cytely handle the segmentation, feature extraction, and visualization. No special acquisition hardware or software required. This keeps you in control of imaging while Cytely augments the downstream analysis, eliminating the need for manual image curation or post hoc guessing. It's the fastest way to turn microscopy into insight.

Close the Loop with Smart Acquisition

Close the Loop with Smart Acquisition

Identify populations of interest through gating and send their coordinates back to the microscope for high-resolution or multi-modal follow-up imaging. Cytely supports a feedback loop known as Data-Driven Acquisition (DDA), allowing you to capture the most informative cells, not just what happens to be in frame. Whether you're capturing rare events or dynamic processes like actin remodeling, Cytely lets you zero in on what matters without scanning every cell manually. This creates a seamless cycle from discovery to deep dive, powered by your own data.

Cytely Advantage

Built for Spatial Single-Cell Discovery

Built for Spatial Single-Cell Discovery

Cytely analyzes individual cells and objects at true single-cell resolution, retaining spatial and subcellular context. This is essential for studying interactions, heterogeneity, and rare phenotypes. From phagocytosis to infection assays, Cytely allows you to visualize object relationships and spatial patterns, unlocking insights not possible with population averages or blind analysis.

AI Powered No-Code Workflow Creation

AI Powered No-Code Workflow Creation

Build complete analysis workflows without writing a single line of code. Pre-trained AI handles segmentation automatically, identifying and profiling cells, debris, and subcellular structures with no parameter tweaking or training data required. From there, gating, feature extraction, and visualization combine into a reusable workflow that feeds directly into downstream analysis, saving time and increasing reproducibility. It also enables direct visual feedback, so you can verify what's being analyzed, not just trust a number.

Visual Gating

Visual Gating

Dive deeper into your data with an image flow cytometry-like experience using data from the initial DIA scan. Plot any measured morphological or intensity features against each other in interactive 2D scatter plots. Interactively gate populations of interest directly on the plots. As you gate, see corresponding cropped images of the selected cells instantly update in one panel, while another panel highlights the gated objects within a zoomable overview image. This allows for intuitive discovery of correlations and insights. The available parameters adapt to your specific assay (e.g., prey intensity/area for phagocytosis, overlapping area for colocalization). Crucially, you can then select gated populations or individual cells and trigger targeted Data-Dependent Acquisition (DDA) to re-image those specific cells at higher resolution or with different modalities.

Share It. Reproduce It. Build on It.

Share It. Reproduce It. Build on It.

Cytely lets you share complete experiments, raw data, gates, images, and plots, in a single secure link. Collaborators, reviewers, or auditors can explore data interactively and verify every step from raw image to final result.

Ready-to-Go Assays

Pre-validated measurement workflows for the assays your team runs every day

Rapid Cell Count & Classification

Rapid Cell Count & Classification

Cytely enables fast quantification of cell populations and contaminants based on morphology and staining. Get immediate counts of target cells, debris, RBCs, or platelets, with no scripting required. This is ideal for QC steps, sample validation, or analyzing cell mixtures where identification and exclusion are key.

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Transfection Efficiency Analysis

Transfection Efficiency Analysis

Identify transfected cells based on intensity thresholds, co-localization, or nuclear vs cytosolic signal distribution. Cytely automatically quantifies double-transfected populations and assesses heterogeneity across the field. Visualize results as scatter plots, histograms, or spatial overlays. Quickly determine the proportion of successful transfection events and explore subgroups.

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And More Assays

And More…

From co-culture assays to rare cell detection, Cytely supports flexible, user-defined workflows built on its robust segmentation and gating foundation. If it's in the image, Cytely can quantify it and let you explore it visually. More assay templates and pre-configured workflows are coming soon, including apoptosis, cell spreading, and synapse formation.

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See Cytely in Action

Preview of shared phagocytosis dataset in Cytely

Explore a High-Resolution Phagocytosis Dataset

See Cytely in action with real phagocytosis data. Interact with the analysis, explore gating, and see what reproducible measurement looks like in practice.

Explore Interactive Dataset

Scientific Foundation

Powered by Data-Driven Microscopy, the peer-reviewed framework behind Cytely's approach to reproducible, automated measurement.

Visual abstract of the Data-Driven Microscopy paper

Cell Rep Methods. 2023 Mar 6;3(3):100419. doi: 10.1016/j.crmeth.2023.100419.

Data-driven microscopy allows for automated context-specific acquisition of high-fidelity image data.

Oscar André, Johannes Kumra Ahnlide, Nils Norlin, Vinay Swaminathan, Pontus Nordenfelt.

Read Full Paper (PMC10088093)

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