Here we look at a basic blood sample stained for nuclei, cytosol, and membrane. We easily gated out RBCs and platelets by their lack of nuclear signal and smaller size, while neutrophils popped out with their strong nuclear and cytosolic staining. Ordinarily, you’d need multiple antibodies or RBC lysis to achieve this separation, but with Cytely, you can do it in a single workflow, preserving your sample’s spatial context and completing the entire analysis in a matter of minutes.
Breaking the Boundaries of Blood Cell Analysis: A One-Minute Revolution with Cytely
In the evolving landscape of cellular analysis, traditional methods often force compromises between speed, specificity, and spatial context. Today, we're showcasing how Cytely transforms this paradigm, enabling researchers to distinguish critical blood cell populations with unprecedented efficiency while preserving their native spatial relationships.
Redefining Blood Cell Classification
The conventional approach to blood cell differentiation typically involves either elaborate antibody panels with flow cytometry or painstaking manual microscopy analysis. Cytely bridges this methodological divide by leveraging basic nuclear and cytosolic markers to rapidly classify neutrophils, red blood cells (RBCs), and platelets—all while maintaining their spatial context within the sample.
This breakthrough capability eliminates the need for:
- Multiple specialized antibodies
- RBC lysis protocols
- Tedious manual segmentation
- Sacrifice of spatial distribution data
The Technical Advantage
What makes Cytely's approach transformative is its integration of high-throughput classification with precise spatial mapping. The platform harnesses interactive gating with real-time visual feedback, allowing researchers to dynamically adjust parameters based on:
- Cell size
- Signal intensity
- Morphological features
- Fluorescence patterns
This flexibility creates a responsive analytical environment that adapts to your specific research questions rather than constraining your investigation to predetermined protocols.
Practical Application: Blood Cell Differentiation in Action
In our demonstration dataset (linked here), we examined a basic blood sample stained with just three fundamental markers:
- Nuclei dye (Alexa 488)
- Cytosolic dye (Alexa 555)
- Membrane dye
The analysis workflow revealed distinct cell populations through a strategic gating sequence:
- Initial Population Separation: Plotting Circularity vs Area identified a distinct cluster with relatively high area
- Neutrophil Isolation: By examining Mean Alexa 488 (nuclei stain) vs Area, we precisely captured cells with clear nuclear signal
- RBC and Platelet Differentiation: Further analysis of the non-nucleated populations using Mean Alexa 555 (cytosolic marker) vs Area distinguished:
- Platelets: Smaller area with lower Alexa 555 signal
- RBCs: Slightly larger area with higher Alexa 555 signal
The entire classification process was completed in approximately one minute—a dramatic efficiency gain compared to conventional methods.
Why This Matters
This approach represents more than just incremental improvement—it's a fundamental shift in how we can approach blood cell analysis. By preserving spatial context while enabling rapid classification, Cytely opens new possibilities for understanding cellular relationships in their native environment.
Researchers across expertise levels can now access sophisticated cellular analysis without specialized equipment or complex protocols, democratizing access to critical insights and accelerating discovery timelines.